|IBV S1 Phylogeny
PCR cycle sequencing is used by our lab to
identify new IBV S1 genotypes. General (= universal)
degenerate primers are used to amplify hypervariable regions that
are used to identify field isolates from commercial poultry flocks.
PCR products approximately 600 bp in length are then sequenced and
aligned with sequences of known reference strains. Sequence
analysis yields phylogenetic tree (= dendogram) and nucleotide and
protein similarity values (%) for the included genotypes. The
phylogenetic tree below illustrates the relationship between
selected genotypes from a variety of geographic locations. The
primers and procedures for IBV PCR cycle sequencing are described in
Kingham, B. F., C. L. Keeler, Jr., W. A. Nix, B. S. Ladman, and J.
Gelb, Jr. Identification of avian infectious bronchitis virus by
direct automated cycle sequencing of the S-1 gene. Avian Diseases.
S1 phylogeny of representative avian infectious bronchitis virus
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North America, Central America (Costa Rica), South America
(Colombia), Europe, Australia,
Middle East (Israel), Africa (Egypt), and India.
Note. Nephropathogenic genotypes include PA/Wolgemuth/98, PA/171/99,
B 1648 (Belgium), and N1/62 (Australia, T strain).
PA = Pennsylvania (USA); DE = Delaware (USA); CV = California
Download sequences from GenBank at http://www.ncbi.nlm.nih.gov/.
(Updated August 2003)